The two spectrograms are in the same dimension.
I tried S = spectrogram() to get the vector S, but I don't know how to draw back to spectrogram with it.
In the documentation, it says that the images are drawn with the command surf(T,F,10*log10(abs(P)));. So for example, you could plot the difference of two of these images with:
%# make some data
T = 0:0.001:2;
X1 = chirp(T,100,1,200,'q');
X2 = chirp(T,150,1,200,'q');
%# plot the first spectrogram
subplot(3,1,1);
spectrogram(X1,128,120,128,1E3);
title('Quadratic chirp 100Hz')
%# plot the second spectrogram
subplot(3,1,2);
spectrogram(X2,128,120,128,1E3);
title('Quadratic chirp 150Hz')
%# plot their difference
subplot(3,1,3);
[~,F1,T1,P1]=spectrogram(X1,128,120,128,1E3);
[~,F2,T2,P2]=spectrogram(X2,128,120,128,1E3);
%# just use the difference of the above two plot's z-values:
surf(T2,F2,10*(log10(abs(P2))-log10(abs(P1))),'edgecolor','none');
%# this is actually a 3D plot, so we set the viewing
%# angle as straight up and rotated by 90 to match the previous plots
view(90,-90);
axis tight;
title('Difference of Plots (2nd - 1st)')
ylabel('Freq (Hz)');
xlabel('Time');
This code makes the following plot:
来源：https://stackoverflow.com/questions/9443854/how-to-do-subtraction-between-2-spectrogram-and-then-draw-a-new-one-in-matlab