Write a couple 10 minute examples and run them in your profiler.
That will tell you which is faster to the millisecond.
If you don't have a profiler, post them here, and I will run them in my PHPEd profiler.
I suspect that much of the time difference, if any, comes from having to open the file that a class is stored in, but that would have to be tested too.
Then ask yourself if you care that much about a few milliseconds vs having to maintain spaghetti code - will any of your users ever notice?
Edit
The profiler won't simulate high traffic volumes, but it will tell you which method is faster for a single user, and which parts of the code are using how much time. Especially if you profile the operations being done repeatedly - say 1000 times each in a loop.
We can assume (though not always) that faster code used by a lot of people will be faster than slower code used by a lot of people.