Another possibility is simply replacing commas with periods in your file, then load the new file in MATLAB.
On Linux or Mac, we can use sed
or tr
UNIX utilities:
$ cat file.dat | tr ',' '.' > file2.dat
On Windows, we can use PowerShell:
PS> gc file.dat | % { $_ -replace ',', '.' } | sc -encoding ascii file2.dat
Eitherway, we can load the new file in MATLAB simply as:
>> load -ascii file2.dat
>> disp(file2)
1.6000 2.0000 6.5000 5.0000
0 1.0000 4.0000 2.5000