When you set the size of a windows form, ie;
Form1.Size = new System.Drawing.Size(700, 500);
Does this include the border which windows put
That depends, you'll get a different size when you target .NET 4.5 for example. The border is always included but you don't really know how much of the border is included. An issue with the fat borders you get with Aero and the skinny ones you get in XP.
It is almost always the wrong thing to do. You always want to assign the ClientSize property instead. Important, it doesn't include the borders so you can be sure that controls still fit.
And hard-coding the size is always wrong as well. Your form will be rescaled, depending on the video adapter's dots-per-inch setting. The larger the DPI setting, the bigger the form needs to be to still fit its content. So the correct statement ought to resemble this:
this.ClientSize = new Size(PictureBox3.Right, PictureBox3.Bottom);
On the assumption that "PictureBox3" is the control in the far right-bottom corner that you want to keep visible. This statement needs to appear in the Load event handler to ensure that rescaling was done, it can't work in the constructor. One of the very few good reasons to use the Load event.