I have made a program that finds and measures the radius of yeast colonies in images. It uses pygame for pixel access and graphical display of the images.
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Fear not! Other people have had the same problem and have been helped.
Also, here are few useful links. These are GUI toolkits for PyGame GooeyPy, Mike's Simple GUI, OcempGUI and a whole lot more.
PGU I think is one of the popular libraries that most game developers use and it seems to be reasonably well maintained.