Does Mac App Store accept application written in Python/Java/Ruby?
The Good News
Yes, you can submit a Java app to the Mac App Store. Not true in the past, but it is true at least as of now in 2013.
One example is the successful MoneyDance personal finance app currently available for sale in the Mac App Store.
The Bad News
Preparing and submitting your app is neither simple nor easy. You have to jump through some hoops.
More Info
By the way, you can use both Swing and JavaFX in your app. JavaFX is now bundled with Java, at least as of Java 7 Update 40 according to these Release Notes.
The newly released Java 9 should make this process of wrapping an app with a runtime even easier and better.
Java 9 brings “modularization”, Java Platform Module System (JPMS), where the approximately 5,000 classes bundled with a JVM are grouped into about a hundred chunks known as modules. You can include only the modules containing classes actually used by your particular app rather hauling along every library. If you don't use JSON or CORBA, then you need not include the JSON or CORBA related modules.
The new jlink tool does this work for you, to assemble and optimize a set of modules and their dependencies into a custom run-time image. As a bonus, some link-time optimization work is performed to make your app launch faster.
These features are supported on 64-bit macOS systems, along with other platforms.
Java 9 also brings new support for native compiling, to build an app optimized for specific hardware. But this ability is experimental, and not yet ready for production use.
Oracle has announced the end of web deployment technology, including Java Web Start, the previously recommended avenue for desktop apps. So now, Oracle expects all Java apps to be delivered bundled with a JVM such as is done with the jlink
tool mentioned above.
So, the process of wrapping an app with a Java runtime that was something of a rarity just for the Apple App Store is now mainstream. Oracle expects any Java-based desktop app to be installed this way.
For Python apps, I wrote a comprehensive article explaining how to build and submit such apps to the Mac App Store. It includes source code and build scripts for a barebones example app that I have successfully submitted.
Possibly Java, if you bundle the Java application into a mac application.
Ruby ? Yes !
Apple already accepted one of my apps written in MacRuby.
Yes and no. Apple doesn't care what language your App is written in, but it cannot depend on or install anything outside of the binary you submit to Apple. The relevant guidelines are below:
2.14
Apps must be packaged and submitted using Apple's packaging technologies included in Xcode - no third party installers allowed
2.15
Apps must be self-contained, single application installation bundles, and cannot install code or resources in shared locations
2.16
Apps that download or install additional code or resources to add functionality or change their primary purpose will be rejected
So, while this means you can't depend on Ruby or Python, you could use a framework like MacRuby to build your app in Ruby and embed the Ruby interpreter. Speaking for MacRuby, apps have been successfully submitted to the Mac App Store this way.
AFAIK, Cocoa will allow you include code written in different languages (including Ruby and Python, but not Java I don't think).
Check the Apple SDK's for more info, but I believe (thanks to Cocoa) it's not a problem.