EDIT: After lots of help and not having a clue what\'s going on, it worked using a different method of opening (see @JohnMuggin\'s help below)--so I un-
And at the very last try this little sub. If it doesn't open your workbook then there is a problem with the path or filename
Sub opendfghj()
Dim wb As Workbook
Workbooks.Open Filename:="C:\Users\User\Desktop\Templates and Example data\Repeat Tests\file.xlsx"
Set wb = ActiveWorkbook
wb.Worksheets("Sheet1").Range("A1") = "It Works"
End Sub
If you have a copy of the workbook open (in a different folder) with the same name as the one your VBA is trying to open, it fails silently. The ActiveWorkbook solution appears to work - as you have at least one workbook open already - and that is active - but its not the one you think it is.
I imagine it it could be a common mistake - as while developing a VBA project you might have a copy of the target workbook open to check on column numbers etc.