I got a complicated problem with ASP.Net 4.0 Ajax....I started a website with Visual Studio 2010 on my machine,and added some update panels they used to work fine,but sudden
I had this problem and sought an answer from the almighty Google, tried various suggestions including the ones above but had no luck. Gave up and moved on to other work, came back a few days later and the problem had disappeared.
I resumed work, made some code changes and published my website and the problem reappeared. Went back to the Google and came across someone who had the problem while using the 3.5 framework. In that case s/he was able to resolve the problem by going to the 'Add/Remove Programs' control panel and selecting the repair option.
I did likewise, repairing the 'MS .NET Framework 4 Client Profile' and 'MS .NET Framework 4 Extended'. That fixed the problem for me.
Hope that solves it for someone else.