How to get the paremeters when I have a given data and formula in r?

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I have a data frame like below,

structure(list(`Plot No.` = c(11, 12, 13, 14, 21, 22, 11, 12, 13, 14, 21, 22, 23, 11, 12, 13, 14, 21, 22, 23), Sample_ID = c(1, 2,


                      
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