remove gradient of a image without a comparison image

Question

currently i am having much difficulty thinking of a good method of removing the gradient from a image i received.

The image is a picture taken by a microscope camera th

Answer 1

I would like to point you to this paper: http://www.cs.berkeley.edu/~ravir/dirtylens.pdf, but, in my opinion, without any sort of calibration/comparison image taken apriori, it is difficult to mine out the ground truth from the flared image.

However, if you are trying to just present the image minus the lens flare, disregarding the actual scientific data behind the flared part, then you switch into the domain of image inpainting. Criminsi's algorithm, as described in this paper: http://research.microsoft.com/pubs/67276/criminisi_tip2004.pdf and explained/simplified in these two links: http://cs.brown.edu/courses/csci1950-g/results/final/eboswort/ http://www.cc.gatech.edu/~sooraj/inpainting/, will do a very good job in restoring texture information to the flared up regions. (If you'd really like to pursue this approach, do mention that. More comprehensive help can be provided for this).

However, given the fact that we're dealing with microscopic data, I doubt if you'd like to lose the scientific data contained in a particular region of an image. In that case, I really think you need to find a workaround to determine the flare model of the flash/light source w.r.t the lens you're using.

I hope someone else can shed more light on this.