If I have a dataframe similar to this one
Apples Bananas Grapes Kiwis
2 3 nan 1
1 3 7 nan
nan nan 2
It is possible to do it without knowing the number of columns and even without iloc:
print(df)
Apples Bananas Grapes Kiwis
0 2.0 3.0 NaN 1.0
1 1.0 3.0 7.0 NaN
2 NaN NaN 2.0 3.0
cols_to_sum = df.columns[ : df.shape[1]-1]
df['Fruit Total'] = df[cols_to_sum].sum(axis=1)
print(df)
Apples Bananas Grapes Kiwis Fruit Total
0 2.0 3.0 NaN 1.0 5.0
1 1.0 3.0 7.0 NaN 11.0
2 NaN NaN 2.0 3.0 2.0