A short description about my working environment: win 7 x64, python 2.7 x64, scrapy 0.22, cx_Freeze 4.3.2.
First, I developed a simple crawl-spider and it works fine
Replace your cx_Freeze code with this.
import sys
from cx_Freeze import setup, Executable
build_exe_options = {"packages": ["os","twisted","scrapy","test"], "excludes": ["tkinter"],"include_msvcr":True}
base = None
setup( name = "MyScript",
version = "0.1",
description = "Demo",
options = {"build_exe": build_exe_options},
executables = [Executable("C:\\MyScript", base=base)])
The difference in code is I have included the whole of the packages so you can access all functions from them.