In Sourcetree you can do this easily via the GUI.
You can check which command Sourcetree uses to unstage a file.
I created a new file and added it to Git. Then I unstaged it using the Sourcetree GUI.
This is the result:
Unstaging files [08/12/15 10:43]
git -c diff.mnemonicprefix=false -c core.quotepath=false -c credential.helper=sourcetree reset -q -- path/to/file/filename.java
Sourcetree uses reset
to unstage new files.