Try this:
Option Explicit
Sub Sample()
Dim Ret
Ret = IsWorkBookOpen("C:\myWork.xlsx")
If Ret = True Then
MsgBox "File is open"
Else
MsgBox "File is Closed"
End If
End Sub
Function IsWorkBookOpen(FileName As String)
Dim ff As Long, ErrNo As Long
On Error Resume Next
ff = FreeFile()
Open FileName For Input Lock Read As #ff
Close ff
ErrNo = Err
On Error GoTo 0
Select Case ErrNo
Case 0: IsWorkBookOpen = False
Case 70: IsWorkBookOpen = True
Case Else: Error ErrNo
End Select
End Function